Related Experiment Video
Updated: Aug 9, 2026

Preparation of Viral DNA from Nucleocapsids
Published on: August 16, 2011
Purification of genomic sized herpesvirus DNA using pulse-field electrophoresis
R J Isfort1, D Robinson, H J Kung
1Genetic Toxicology Section, Procter and Gamble Company, Cincinnati, OH 45239.
Abstract:
A new method for the separation of genomic sized herpesvirus DNA was developed. This method utilizes the improved separation of extremely high molecular weight DNA molecules by pulse-field electrophoresis. This method has allowed us to separate and purify herpesvirus DNA (greater than 100 kbp) free of cellular DNA, directly from infected cells. In the case of cell associated herpesviruses (such as Marek's disease herpesvirus), this method is more efficient than present isolation procedures. The DNA obtained is able to be restriction enzyme digested and used in cloning experiments.
Related Concept Videos
DNA Isolation
DNA Agarose Gel Electrophoresis
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
Herpes

