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Related Experiment Video

Updated: Jun 1, 2026

Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry
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Cyclo-linopeptide A methanol solvate.

J Wilson Quail, J Shen, M J T Reaney

    Acta Crystallographica. Section E, Structure Reports Online
    |May 18, 2011
    PubMed
    Summary
    This summary is machine-generated.

    Researchers crystallized cyclo-linopeptide A (CLP-A), a nine-peptide polypeptide from flax oil. This cyclic peptide forms chains linked by hydrogen bonds, with methanol molecules integrated into the crystal structure.

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    An Efficient Method for the Synthesis of Peptoids with Mixed Lysine-type/Arginine-type Monomers and Evaluation of Their Anti-leishmanial Activity

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    Area of Science:

    • Biochemistry and Structural Biology
    • Natural Product Chemistry
    • Crystallography

    Background:

    • Cyclic peptides are complex biomolecules with diverse biological activities.
    • Flax oil is a source of natural products, including potentially novel peptides.
    • Understanding the solid-state structure of peptides is crucial for their characterization and potential applications.

    Purpose of the Study:

    • To determine the crystal structure of a nine-peptide polypeptide isolated from flax oil.
    • To elucidate the self-assembly and intermolecular interactions of this cyclic peptide in the solid state.
    • To characterize the role of the methanol solvate in the crystal packing.

    Main Methods:

    • Isolation and purification of the cyclic peptide from flax oil.
    • Single crystal X-ray diffraction analysis to determine the molecular and crystal structure.
    • Analysis of hydrogen bonding networks and crystal packing.

    Main Results:

    • Crystals of cyclo-(Pro-Pro-Phe-Phe-Leu-Ile-Ile-Leu-Val), the methanol solvate of cyclo-linopeptide A (CLP-A), were successfully obtained.
    • CLP-A molecules form extended chains along the a-axis through N-H⋯O hydrogen bonds.
    • Methanol molecules act as hydrogen bond acceptors and donors, interacting with CLP-A molecules and contributing to the overall crystal lattice.

    Conclusions:

    • The crystal structure reveals a specific hydrogen-bonding motif driving the self-assembly of CLP-A.
    • The methanol solvate plays an integral role in stabilizing the crystal structure of CLP-A.
    • This study provides valuable structural insights into a novel cyclic peptide from a natural source.