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Isolation and characterization of microsatellite markers in Acca sellowiana (Berg) Burret
K L Santos1, M O Santos, P R Laborda
1Programa de Pós-Graduação em Recursos Genéticos Vegetais, Departamento de Fitotecnia, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina, CP 476, CEP 88040-900, Florianópolis, Santa Catarina, Brazil, Programa de Pós-Graduação em Genética e Biologia Molecular, Instituto de Biologia, Universidade Estadual de Campinas, São Paulo, Brazil.
Abstract:
Acca sellowiana has commercial potential due to the quality and the unique flavor of its fruit. Conservation of natural populations and management of breeding programmes would benefit from the availability of molecular markers that could be used to characterize levels and distribution of genetic variability. Thus, 13 microsatellite markers were developed from an enriched genomic library of A. sellowiana. They were characterized using 40 samples. The expected and observed heterozygosities ranged from 0.513 to 0.913 and from 0.200 to 0.889, respectively. These are the first microsatellite loci characterized from A. sellowiana that will contribute to improve researches on its genetic conservation, characterization and breeding.
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