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Updated: Jun 1, 2026
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A High-content Imaging Workflow to Study Grb2 Signaling Complexes by Expression Cloning
Published on: October 30, 2012
Problem-solving test: Expression cloning of the erythropoietin receptor
1Department of Medical Biology, Medical School, University of Pécs, H-7624 Pécs, Hungary. jozsef.szeberenyi@aok.pte.hu.
Abstract:
Terms to be familiar with before you start to solve the test: cytokines, cytokine receptors, cDNA library, cDNA synthesis, poly(A)⁺ RNA, primer, template, reverse transcriptase, restriction endonucleases, cohesive ends, expression vector, promoter, Shine-Dalgarno sequence, poly(A) signal, DNA helicase, DNA ligase, topoisomerases, [¹²⁵I] labeling, transfection, mock transfection, SDS-polyacrylamide gel electrophoresis, β-mercaptoethanol, autoradiography.
