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MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Jun 1, 2026

mirMachine: A One-Stop Shop for Plant miRNA Annotation

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

mirAct: a web tool for evaluating microRNA activity based on gene expression data.

Zhi Liang¹, Hong Zhou, Zongxiao He

¹Hefei National Laboratory for Physical Sciences at Microscale and School of Life Science, University of Science and Technology of China, 96 Jinzhai Road, Hefei, Anhui 230027, China. liangzhi@ustc.edu.cn

Nucleic Acids Research

|May 21, 2011

Summary

This summary is machine-generated.

The mirAct web server analyzes gene expression data to reveal microRNA (miRNA) activity, aiding in understanding cellular regulation. This tool offers clustering analysis for complex biological networks.

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Last Updated: Jun 1, 2026

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Published on: May 1, 2021

Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice

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Published on: August 21, 2019

Area of Science:

Bioinformatics
Computational Biology
Molecular Biology

Background:

MicroRNAs (miRNAs) are key regulators in cellular networks.
Understanding miRNA activity is crucial for deciphering gene regulation.

Purpose of the Study:

To introduce the mirAct web server, a tool for investigating miRNA activity.
To enable analysis of miRNA-gene interactions using gene-expression data.

Main Methods:

Utilizes the negative regulatory relationship between miRNAs and target genes.
Supports multiple-class gene-expression data.
Incorporates clustering analysis based on computed miRNA activity.

Main Results:

The mirAct web server provides a framework for miRNA activity investigation.
Demonstrates performance through comparison with similar bioinformatics tools.
Illustrates practical applications via case studies.

Conclusions:

mirAct is a valuable tool for studying miRNA functions in biological systems.
Facilitates deeper insights into gene regulatory networks through miRNA activity analysis.