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Related Concept Videos

RNA Stability01:53

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
RNA Stability01:53

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
Nucleic Acid Structure01:25

Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA has a double-helix structure. The...
Nucleic Acids02:43

Nucleic Acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
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The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...
Nucleic acids02:43

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RNA Structure01:19

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The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
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Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation
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Published on: February 12, 2022

Covalent stabilization of a small molecule-RNA complex.

Hayden Peacock1, Radhika Bachu, Peter A Beal

  • 1Department of Chemistry, University of California, One Shields Ave, Davis, CA 95616, United States.

Bioorganic & Medicinal Chemistry Letters
|May 24, 2011
PubMed
Summary

Researchers formed a covalent bond between RNA aptamers and helix-threading peptides (HTPs). This reaction, dependent on the aptamer

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Published on: January 3, 2019

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Chemical Biology

Background:

  • RNA aptamers are crucial for molecular recognition and therapeutics.
  • Helix-threading peptides (HTPs) offer unique structural motifs for protein-RNA interactions.
  • Covalent modification strategies are essential for studying and stabilizing biomolecular complexes.

Purpose of the Study:

  • To demonstrate and characterize covalent bond formation between RNA aptamers and HTPs.
  • To investigate the mechanism and specificity of the observed covalent reaction.
  • To establish a method for creating stable HTP-RNA complexes for structural and discovery purposes.

Main Methods:

  • Synthesis of RNA aptamers with cysteamine-tethered nucleobases.
  • Preparation of HTPs with N-terminal α-bromoacetamide modifications.
  • Performing covalent cross-linking reactions between aptamers and HTPs.
  • Inhibition assays using complementary DNA strands to assess reaction specificity.

Main Results:

  • Successful high-yield covalent bond formation between modified RNA aptamers and HTPs.
  • Demonstration that the covalent reaction is dependent on the aptamer's specific binding site for HTPs.
  • Inhibition of the reaction by a DNA strand complementary to the aptamer, confirming site-specific binding.

Conclusions:

  • A robust method for covalently linking RNA aptamers and HTPs has been established.
  • This covalent tethering strategy is sequence-specific and relies on the aptamer's binding pocket.
  • The findings facilitate structural studies of HTP-RNA complexes and the discovery of novel high-affinity aptamer analogs.