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Related Concept Videos

Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Mass Spectrometers01:16

Mass Spectrometers

This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
Mass Spectrum: Interpretation01:24

Mass Spectrum: Interpretation

An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a soft-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.To...
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
Molecular Spectroscopy: Absorption and Emission01:14

Molecular Spectroscopy: Absorption and Emission

Molecules possess discrete energy levels called quantum states. Unlike atoms, which have simpler energy levels, molecules possess additional rotational and vibrational energy levels. Each energy level is separated by an energy gap, with the gaps between adjacent electronic, vibrational, and rotational levels varying significantly. The three types of energy levels in a diatomic molecule are shown in Figure 1.

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Updated: Jun 1, 2026

Correlative Optical Spectroscopy and Mass Spectrometry Imaging Methodology to Visualise Drug Distribution in a Soft Tissue Section
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Correlative Optical Spectroscopy and Mass Spectrometry Imaging Methodology to Visualise Drug Distribution in a Soft Tissue Section

Published on: June 20, 2025

Molecular SPECT Imaging: An Overview.

Magdy M Khalil1, Jordi L Tremoleda, Tamer B Bayomy

  • 1Biological Imaging Centre, MRC Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Hospital Campus, Du Cane Road, London W12 0NN, UK.

International Journal of Molecular Imaging
|May 24, 2011
PubMed
Summary
This summary is machine-generated.

Single photon emission computed tomography (SPECT) offers advanced molecular imaging for personalized medicine. This review details SPECT technology, radiopharmaceuticals, and its applications in disease detection and diagnosis.

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Atmospheric-pressure Molecular Imaging of Biological Tissues and Biofilms by LAESI Mass Spectrometry
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Atmospheric-pressure Molecular Imaging of Biological Tissues and Biofilms by LAESI Mass Spectrometry

Published on: September 3, 2010

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Last Updated: Jun 1, 2026

Correlative Optical Spectroscopy and Mass Spectrometry Imaging Methodology to Visualise Drug Distribution in a Soft Tissue Section
07:05

Correlative Optical Spectroscopy and Mass Spectrometry Imaging Methodology to Visualise Drug Distribution in a Soft Tissue Section

Published on: June 20, 2025

Atmospheric-pressure Molecular Imaging of Biological Tissues and Biofilms by LAESI Mass Spectrometry
09:22

Atmospheric-pressure Molecular Imaging of Biological Tissues and Biofilms by LAESI Mass Spectrometry

Published on: September 3, 2010

Area of Science:

  • Medical Imaging
  • Nuclear Medicine
  • Molecular Diagnostics

Background:

  • Molecular imaging has rapidly advanced, with significant investment in new technologies and pharmaceuticals.
  • Single photon emission computed tomography (SPECT) and positron emission tomography (PET) are key modalities driving personalized medicine.

Purpose of the Study:

  • To review the current standing of molecular SPECT imaging.
  • To compare SPECT with other imaging techniques, highlighting its strengths and weaknesses.
  • To discuss different SPECT designs, detection systems, radiopharmaceuticals, and applications.

Main Methods:

  • Review of current literature on SPECT technology and applications.
  • Comparative analysis of SPECT and PET imaging principles.
  • Overview of radiopharmaceutical agents for SPECT.

Main Results:

  • SPECT imaging provides valuable molecular insights for diagnostics.
  • Discussion of various SPECT hardware designs and their performance characteristics.
  • Enumeration of radiopharmaceutical agents and their clinical/preclinical relevance.

Conclusions:

  • SPECT remains a crucial tool in molecular imaging and personalized medicine.
  • Understanding SPECT's capabilities and limitations is essential for its optimal use.
  • SPECT imaging demonstrates broad applicability in disease detection and diagnosis across various medical fields.