Related Experiment Video
Updated: Jun 1, 2026
Analysis of HBV-Specific CD4 T-cell Responses and Identification of HLA-DR-Restricted CD4 T-Cell Epitopes Based on a Peptide Matrix
Published on: October 20, 2021
[Study on HLA-DRB1 alleles in patients with familial hepatitis B in Shanxi]
Xiao-Yu He1, Liao-Yun Zhang, Xiao-Hong Zhang
1Department of Infectious Diseases, First Clinical Medical College, Shanxi Medical University, Taiyuan 030001, China.
Insights
Human Leukocyte Antigen (HLA)-DRB1*0701 is linked to increased susceptibility to familial hepatitis B. Conversely, HLA-DRB1*04 and HLA-DRB1*13 alleles may offer resistance to the condition.
Area of Science:
- Immunogenetics
- Hepatology
- Molecular Biology
Context:
- Familial hepatitis B represents a significant public health concern.
- Understanding the genetic factors influencing hepatitis B virus (HBV) infection is crucial for developing targeted interventions.
- Human Leukocyte Antigen (HLA) genes play a critical role in immune response and disease susceptibility.
Purpose:
- To investigate the association between specific Human Leukocyte Antigen (HLA)-DRB1 alleles and the susceptibility to familial hepatitis B.
- To identify potential genetic markers that may predict the outcome of hepatitis B virus (HBV) infection within families.
Summary:
- A study involving 151 individuals from familial hepatitis B families utilized Polymerase Chain Reaction-Sequence Specific Oligonucleotide Probing (PCR-SSOP) to analyze HLA-DRB1 alleles.
- Results indicated a significantly higher frequency of HLA-DRB1*0701 in the chronic hepatitis B (CHB) group compared to controls (17.8% vs 7.4%, P < 0.05).
- Conversely, HLA-DRB1*0401/0403/0405 and HLA-DRB1*1301/1302 alleles were more prevalent in the control group, suggesting a protective role against CHB (P < 0.05).
Impact:
- The findings suggest that HLA-DRB1*0701 may act as a susceptibility gene for familial hepatitis B.
- HLA-DRB1*04 and HLA-DRB1*13 alleles appear to confer resistance to familial hepatitis B, potentially regulating HBV infection outcomes.
- This research provides valuable insights into the immunogenetic basis of hepatitis B and could inform future genetic screening and personalized treatment strategies.
Objective:
To study the association between HLA-DRB1 alleles and familial hepatitis B.
Methods:
HLA-DRB1 alleles of 151 people in the familial hepatitis B families were detected,by using the the polymerase chain reaction-sequence specific oligonucleotide probing (PCR-SSOP) technique.
Results:
The allele frequency of HLA-DRB1 * 0701 in the chronic hepatitis B (CHB) group was markedly higher than that in the normal control group (17.8% vs 7.4%), with significant correlation between them (P < 0.05, A = 1/0.367 approximately 2.725). The allele frequencies of HLA-DRB1 * 0401/0403/0405 and HLA-DRB1 * 1301/1302 in the normal control group (16.2%, 4.4% ) were markedly higher than that in the CHB group (5.1%, 0), with statistical significance (P < 0.05, A = 3.602; P < 0.05). The other alleles between the CHB group and the normal control group are no significant differences.
Conclusion:
HLA-DRB1 * 0701 is closely associated with the susceptibility to familial hepatitis B,and may be the susceptible or linkage gene. HLA-DRB1* 0401/0403/0405 and HLA-DRB1 * 1301/1302,in inverse proportion to the susceptibility to familial hepatitis B, may be the resistant gene. They may regulate the outcome of familial HBV infection in a patient.
