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Articles linked to this work by shared authors, journal, and citation graph.

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Same author

[Hemophagocytic Syndrome Secondary to Human Parvovirus B19 Infection in an Acquired Immunodeficiency Syndrome Patient:Report of One Case].

Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae·2023
Same author

Repeated COVID-19 relapse during post-discharge surveillance with viral shedding lasting for 67 days in a recovered patient infected with SARS-CoV-2.

Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi·2020
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Positive SARS-CoV-2 RNA recurs repeatedly in a case recovered from COVID-19: dynamic results from 108 days of follow-up.

Pathogens and disease·2020
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[Analysis of the test results of HBV serum markers and HBV DNA of the neonates born to HBsAg-positive mothers].

Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology·2012
Same author

[Efficacy and safety of lamivudine plus adefovir combination therapy and entecavir monotherapy for chronic hepatitis B patients].

Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology·2011
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[Detection of peripheral blood HBV-LHBs transactivation function and its relationship with anti-viral efficacy].

Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology·2010

Related Experiment Video

Updated: Jun 1, 2026

Detection of Invasive Pulmonary Aspergillosis in Haematological Malignancy Patients by using Lateral-flow Technology
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[Clinical application studies on AFP-L3 detected by micro-spin column method].

Jin Gao1, Ai-Fang Xu, Hong-Ying Zheng

  • 1The Sixth People Hospital of Hangzhou, Hangzhou 310014, China.

Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi = Zhonghua Shiyan He Linchuang Bingduxue Zazhi = Chinese Journal of Experimental and Clinical Virology
|May 25, 2011
PubMed
Summary

Lens culinaris agglutinin-reactive alpha-fetoprotein (AFP-L3) aids in distinguishing benign from malignant liver diseases. This biomarker shows significant value for early hepatocellular carcinoma (HCC) detection and diagnosis.

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Area of Science:

  • Hepatology
  • Biochemistry
  • Oncology

Context:

  • Liver diseases encompass a spectrum from benign conditions to malignant hepatocellular carcinoma (HCC).
  • Accurate differentiation is crucial for timely and appropriate patient management.
  • Early detection of HCC significantly improves patient outcomes.

Purpose:

  • To evaluate the clinical utility of Lens culinaris agglutinin-reactive alpha-fetoprotein (AFP-L3) in differentiating benign and malignant liver diseases.
  • To assess the role of AFP-L3 as an early warning marker for hepatocellular carcinoma (HCC).

Summary:

  • Alpha-fetoprotein (AFP) variants were isolated using Lens culinaris agglutinin (LCA) micro-spin columns from 300 liver disease patients.
  • AFP and AFP-L3 levels were measured via electrochemical luminescence (ECL), calculating AFP-L3 proportions.
  • AFP-L3 positive rates were significantly higher in HCC patients (95%) compared to chronic liver disease patients (64%) (P < 0.01).
  • AFP-L3 positivity in chronic liver disease patients correlated with a significantly higher incidence of HCC (P < 0.01).
  • No significant correlation was found between AFP-L3 proportion and overall AFP levels (r = 0.046, P > 0.05).

Impact:

  • The micro-spin column assay for AFP-L3 demonstrates significant clinical value.
  • This method aids in the differential diagnosis of benign versus malignant liver conditions.
  • AFP-L3 serves as a valuable tool for the early detection and warning of hepatocellular carcinoma.