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Microstructured Devices for Optimized Microinjection and Imaging of Zebrafish Larvae

Microstructured Devices for Optimized Microinjection and Imaging of Zebrafish Larvae

Published on: December 8, 2017

Image-based fluidic sorting system for automated Zebrafish egg sorting into multiwell plates.

Siegfried F Graf¹, Sebastian Hötzel, Urban Liebel

¹Microfluidics & Liquid Handling, Centre Suisse d'Électronique et de Microtechnique, Alpnach, Switzerland.

Journal of Laboratory Automation

|May 26, 2011

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Zebrafish eggs are a promising alternative to animal testing for high-throughput screening (HTS). A new system efficiently places individual Zebrafish eggs into multiwell plates, reducing costs and saving researchers time.

Area of Science:

Biotechnology
Toxicology
Zebrafish Research

Background:

Growing demand to reduce animal testing in scientific research.
Zebrafish eggs/larvae offer a viable alternative for toxicity testing and high-throughput screening (HTS).
Current methods for placing individual Zebrafish eggs into multiwell plates (MWPs) are a bottleneck for HTS.

Purpose of the Study:

To develop a cost-efficient system for automated, individual placement of Zebrafish eggs into MWPs.
To improve the efficiency and reproducibility of HTS using Zebrafish models.
To reduce the manual labor involved in preparing Zebrafish for HTS experiments.

Main Methods:

Development of a novel system for storing, sorting, and placing individual Zebrafish eggs.

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Testing the system's speed and efficiency in filling 96-well plates.

Assessing the survival rates of Zebrafish eggs processed by the system compared to controls.

Evaluating the system's capability with both fertilized and dechorionated eggs.

Main Results:

The system successfully places individual Zebrafish eggs into MWPs with high reproducibility.
A complete 96-well plate can be filled in approximately 11 minutes (8 seconds per egg).
Survival rates of Zebrafish eggs using the system were comparable to control groups.
The system demonstrated effectiveness with both fertilized and dechorionated eggs.

Conclusions:

The developed system is gentle, reliable, and cost-efficient for preparing Zebrafish eggs for HTS.
This automation significantly reduces the manual workload for scientists, allowing more focus on experimental design and analysis.
The system enhances the potential for higher statistical relevance in Zebrafish-based research and toxicity testing.