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Published on: November 13, 2021
A novel liquid-phase piezoelectric immunosensor for detecting Schistosoma japonicum circulating antigen
Zhili Wen1, Shiping Wang, Zhaoyang Wu
1Infectious Disease Department, Nanchang NO.1 Hospital ( NO.3 affiliated hospital of Nanchang University), Nanchang, China. wenzhili@126.com
Aims:
A new liquid-phase piezoelectric immunosensor (LP-PEIS), which can detect Schistosoma japonicum (Sj) circulating antigens (SjCAg) quantificationally, was developed.
Methods:
The IgG antibodies were purified from the sera of rabbits which had been infected or immunized by Sj and were immobilized on the surface of piezoelectric quartz crystal in LP-PEIS by staphylococcal protein A (SPA). It was used to detect SjCAg in sera of rabbits which had been infected by Sj in order to acquire some optimum conditions for detecting SjCAg. Finally, the LP-PEIS with optimum conditions was used to detect SjCAg in sera of patients who had been infected by Sj, and was compared with sandwich ELISA.
Results:
A lot of optimum conditions of LP-PEIS for detecting SjCAg had been acquired. In the detection of patients' sera with acute Schistosomiasis, LP-PEIS has higher positive rate (100%) and lower false positive rate (3.0%) than sandwich ELISA (92.8%, 6.0%). However, there were no significant difference between LP-PEIS and sandwich ELISA.
Conclusions:
LP-PEIS can quantificationally detect SjCAg in patients' sera as well as sandwich ELISA.
