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Related Concept Videos

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

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Related Experiment Video

Updated: Jun 1, 2026

Isolation of Mouse Megakaryocyte Progenitors
10:30

Isolation of Mouse Megakaryocyte Progenitors

Published on: May 20, 2021

Mammalian Cell Sorting using μFACS.

Sung Hwan Cho1, Chun Hao Chen, Frank S Tsai

  • 1Materials Science & Engineering Program, Jacobs School of Engineering, University of California at San Diego, La Jolla, CA, USA.

Conference on Lasers and Electro-Optics : (CLEO). Conference on Lasers and Electro-Optics
|May 27, 2011
PubMed
Summary
This summary is machine-generated.

This study demonstrates a microfabricated fluorescence-activated cell sorter (microFACS) for efficient K562 mammalian cell purification. The microFACS achieved high throughput and significant cell enrichment.

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Last Updated: Jun 1, 2026

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Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Microfluidics

Background:

  • Mammalian cell sorting is crucial for biological research and diagnostics.
  • Traditional fluorescence-activated cell sorters (FACS) can be bulky and expensive.
  • There is a need for miniaturized and high-throughput cell sorting technologies.

Purpose of the Study:

  • To develop and evaluate a highly integrated microfabricated fluorescence-activated cell sorter (microFACS).
  • To assess the performance of the microFACS for purifying K562 mammalian cells.
  • To demonstrate high-throughput cell sorting capabilities.

Main Methods:

  • Utilized a microfluidic chip for cell handling and sorting.
  • Integrated fluorescence detection and sorting mechanisms on a microfabricated platform.
  • Employed K562 mammalian cells as the model system for sorting experiments.

Main Results:

  • Achieved efficient purification of K562 mammalian cells.
  • Demonstrated a high enrichment factor of 230 for the sorted cells.
  • Reached a high throughput exceeding 1,000 cells per second.

Conclusions:

  • The developed microFACS offers a powerful tool for high-purity mammalian cell isolation.
  • Microfluidic cell sorting technology enables rapid and efficient cell purification.
  • This integrated microFACS system has potential applications in various biological and medical fields.