Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An evaluation of brain volume and cortical thickness measurement at 0.55 T.

Magma (New York, N.Y.)·2026
Same author

Denoising of ASL Data Using Deep Learning Priors Generated From Distribution Remapping.

Magnetic resonance in medicine·2026
Same author

Multiplexed magnetic resonance imaging.

Nature·2026
Same author

The "State of the Art" in MR Image Reconstruction? Knowledge, Culture, and What We Leave Behind in An Era of Big Data and Machine Learning.

Magnetic resonance in medicine·2026
Same author

Well-designed k-space coverage is important for good MRI denoising.

Magma (New York, N.Y.)·2026
Same author

Dual association patterns between microglial activation and neuronal health in Alzheimer's disease: a whole-brain MRSI/PET study.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2026
Same journal

SleepConFormer: A Single-Channel EEG Framework for Sleep Staging and Consciousness Assessment in Patients with Disorders of Consciousness.

IEEE transactions on bio-medical engineering·2026
Same journal

Modeling Partial and Total Support of Left Ventricular Assist Device for Discrete Hemodynamic Control Framework.

IEEE transactions on bio-medical engineering·2026
Same journal

A Low-Cost Wearable TI-TACS Stimulator With Bipolar Quadratic-Boost Converter for Current Stimulation Validation in the Rat Brain.

IEEE transactions on bio-medical engineering·2026
Same journal

EMG-Based Gait Estimation Using Koopman-Inspired Method.

IEEE transactions on bio-medical engineering·2026
Same journal

Soft Everting Robots for Medical Applications: A Review.

IEEE transactions on bio-medical engineering·2026
Same journal

Arterial spin labeling cerebral blood flow quantification from quantitative transport mapping based on multiscale fluid mechanics simulation and deep learning.

IEEE transactions on bio-medical engineering·2026
See all related articles

Related Experiment Video

Updated: Jun 1, 2026

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

Deconvolved spatial light interference microscopy for live cell imaging.

Justin P Haldar1, Zhuo Wang, Gabriel Popescu

  • 1Department of Electrical and Computer Engineering and the Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. haldar@uiuc.edu

IEEE Transactions on Bio-Medical Engineering
|May 31, 2011
PubMed
Summary
This summary is machine-generated.

This study introduces a new constrained deconvolution method to enhance resolution in spatial light interference microscopy (SLIM) for label-free live cell imaging. The technique significantly improves image resolution and contrast, aiding in detailed cellular studies.

More Related Videos

Live Images of GLUT4 Protein Trafficking in Mouse Primary Hypothalamic Neurons Using Deconvolution Microscopy
08:47

Live Images of GLUT4 Protein Trafficking in Mouse Primary Hypothalamic Neurons Using Deconvolution Microscopy

Published on: December 7, 2017

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment
07:12

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Published on: January 6, 2026

Related Experiment Videos

Last Updated: Jun 1, 2026

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

Live Images of GLUT4 Protein Trafficking in Mouse Primary Hypothalamic Neurons Using Deconvolution Microscopy
08:47

Live Images of GLUT4 Protein Trafficking in Mouse Primary Hypothalamic Neurons Using Deconvolution Microscopy

Published on: December 7, 2017

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment
07:12

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Published on: January 6, 2026

Area of Science:

  • Biophysics
  • Cell Biology
  • Microscopy

Background:

  • Spatial Light Interference Microscopy (SLIM) offers label-free live cell imaging.
  • Conventional SLIM resolution is limited by diffraction and aberrations.
  • Quantitative optical path length serves as endogenous contrast in SLIM.

Purpose of the Study:

  • To develop a novel constrained deconvolution method for enhancing SLIM resolution.
  • To improve the contrast and resolution of SLIM images for biological studies.

Main Methods:

  • Experimental measurement of the system's point-spread function.
  • Modeling of coherent image formation specific to SLIM.
  • Application of a constrained deconvolution algorithm to SLIM data.

Main Results:

  • Demonstrated significant improvements in spatial resolution.
  • Showcased enhanced contrast in SLIM images.
  • Validated the method using both simulated and experimental data.

Conclusions:

  • The constrained deconvolution method effectively improves SLIM resolution and contrast.
  • This technique is valuable for high-resolution, label-free imaging of cells and subcellular structures.
  • The method facilitates advanced studies of dynamic biological processes.