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Updated: Jun 1, 2026

OLIgo Mass Profiling (OLIMP) of Extracellular Polysaccharides
Published on: June 20, 2010
Flow cytometric analysis of lectin-oligosaccharide interactions by using fluorescent oligosaccharide probes
Tomohisa Kato1, Kenichi Hatanaka
1Japan Chemical Innovation and Inspection Institute, 2-11-9 Iwamotocho, Chiyoda-ku, Tokyo 101-0032, Japan. tkato@iis.u-tokyo.ac.jp
Abstract:
Tetraphenylethylene (TPE) derivatives have strong fluorescence in aggregated state. We report here an oligosaccharide binding assay system using tetraphenylethylene derivatives bearing oligosaccharides with aggregation-induced emission (AIE) characteristics. A tetraphenylethylene derivative bearing 6'-sialyllactose (6'SL) bound to microbeads coated with SSA lectin more effectively than RCA120 lectin. Microbeads that bound to fluorescent oligosaccharide probes could be detected by flow cytometric analysis. Tetraphenylethylene derivatives bearing oligosaccharides are useful for flow cytometric analysis of lectin-oligosaccharide interactions.
