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Published on: January 22, 2020
[Human chorionic gonadotrophin on the expression of epidermal growth factor in rat renal tissues]
Lu-jia Zhang1, Xiu-meng Chen, Rujian Cui
1Department of Nephrology, 1st Affiliated Hospital of Guangzhou Medical College, Guangzhou 510120, China. lucasays@gmail.com
Aim:
To investigate hCG on the expression of epidermal growth factor (EGF) in renal tissues.
Methods:
20 male SD rats weighed 300 - 350 g were injected hCG 200 IU/d for 7 days, and 20 male rats were injected sa-line at equal volume as a control. Kidney tissues were per-formed with immunohistochemical staining to evaluate the distribution of EGF and quantitative PCR (Q-PCR) was used to evaluate the EGF mRNA.
Results:
Immunohistochemtry showed that the positive rate of EGF in experimental group was significantly higher than that in control group(P<0.01), but Q-PCR results showed no significant difference between two groups ( P > 0. 05).
Conclusion:
hCG increased the concentration of EGF by maturing pre-pro-EGF not expressing EGF from de novo synthesis.
