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MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Mass Spectrometry: Complex Analysis01:21

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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
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Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...
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Mass Spectrometric Analysis of Glycosphingolipid Antigens
13:09

Mass Spectrometric Analysis of Glycosphingolipid Antigens

Published on: April 16, 2013

Characterizing oligosaccharides using injected-ion mobility/mass spectrometry.

Y Liu1, D E Clemmer

  • 1Department of Chemistry, Indiana University Bloomington, Indiana 47405.

Analytical Chemistry
|June 7, 2011
PubMed
Summary
This summary is machine-generated.

Ion mobility-mass spectrometry differentiates sugar isomers like raffinose and cyclodextrins. This technique analyzes ion fragmentation patterns to distinguish structural isomers with identical mass-to-charge ratios.

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Physical Chemistry

Background:

  • Ion mobility spectrometry coupled with mass spectrometry (IM-MS) is a powerful analytical technique.
  • Characterizing carbohydrates, such as oligosaccharides and cyclodextrins, presents challenges due to their structural complexity and potential for isomerism.

Purpose of the Study:

  • To measure reduced ion mobilities of negatively charged raffinose, melezitose, and various cyclodextrins using IM-MS.
  • To investigate the fragmentation pathways of these carbohydrates under different injection energies.
  • To demonstrate the utility of IM-MS in distinguishing between isomeric carbohydrate ions.

Main Methods:

  • Electrospray ionization (ESI) was used to generate ions from raffinose, melezitose, and cyclodextrins.
  • Injected-ion mobility/mass spectrometry was employed to measure reduced ion mobilities.
  • Experiments were conducted at both low and high ion injection energies to induce different fragmentation patterns.

Main Results:

  • At low injection energies, deprotonated parent ions were dominant for all analytes.
  • At high injection energies, cross-ring cleavage fragments were observed for cyclodextrins and raffinose.
  • Melezitose exhibited dissociation via glycosidic bond cleavage.
  • Ion mobility distributions successfully differentiated between isomeric parent and fragment ions with the same m/z.

Conclusions:

  • IM-MS is effective in distinguishing structural isomers of carbohydrates.
  • Fragmentation patterns under varying energies provide valuable structural information.
  • The technique offers a pathway for detailed characterization of complex carbohydrate mixtures.