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Investigating carbohydrate isomers by IMS-CID-IMS-MS: precursor and fragment ion cross-sections
M M Gaye1, R Kurulugama, D E Clemmer
1Department of Chemistry, Indiana University, Bloomington, IN 47405, USA. clemmer@indiana.edu.
The Analyst
|August 27, 2015
Summary
Ion mobility spectrometry (IMS) combined with mass spectrometry (MS) differentiates carbohydrate isomers by analyzing fragment ion structures. This technique provides distinct collision cross-section values for isomeric fragments, aiding in precursor identification.
Area of Science:
- Analytical Chemistry
- Carbohydrate Chemistry
- Structural Biology
Background:
- Distinguishing carbohydrate isomers is challenging due to similar physical properties.
- Ion mobility spectrometry-mass spectrometry (IMS-MS) is a powerful tool for analyzing molecular structures.
Purpose of the Study:
- To investigate the structures of singly-lithiated carbohydrate isomers using IMS-MS.
- To evaluate the utility of collision cross-section (ccs) values of fragment ions for isomer differentiation.
Main Methods:
- Utilized ion mobility spectrometry-ion mobility spectrometry-collision-induced dissociation-mass spectrometry (IMS-CID-IMS-MS).
- Analyzed precursor and fragment ions of various monosaccharides, disaccharides, and trisaccharides.
- Measured collision cross-section (ccs) values in Helium.
Main Results:
- IMS-MS of precursor ions showed similar ccs values for most isobaric carbohydrates.
- IMS-CID-IMS-MS analysis yielded distinct ccs values for isomeric fragment ions.
- Reported ccs values for numerous mono-, di-, and trisaccharide isomers.
Conclusions:
- Fragment ion ccs values obtained via IMS-CID-IMS-MS are effective for distinguishing carbohydrate isomers.
- This method offers a valuable approach for structural characterization of complex carbohydrates.
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