Automatic miniaturized fluorometric flow system for chemical and toxicological control of glibenclamide
David S M Ribeiro1, João A V Prior, Christian J M Taveira
1Requimte, Department of Chemical Sciences, Laboratory of Analytical Chemistry and Physical-Chemistry, Faculty of Pharmacy, University of Porto, Rua Aníbal Cunha n° 164, 4099-030, Porto, Portugal.
Abstract:
In this work, and for the first time, it was developed an automatic and fast screening miniaturized flow system for the toxicological control of glibenclamide in beverages, with application in forensic laboratory investigations, and also, for the chemical control of commercially available pharmaceutical formulations. The automatic system exploited the multipumping flow (MPFS) concept and allowed the implementation of a new glibenclamide determination method based on the fluorometric monitoring of the drug in acidic medium (λ(ex)=301 nm; λ(em)=404 nm), in the presence of an anionic surfactant (SDS), promoting an organized micellar medium to enhance the fluorometric measurements. The developed approach assured good recoveries in the analysis of five spiked alcoholic beverages. Additionally, a good agreement was verified when comparing the results obtained in the determination of glibenclamide in five commercial pharmaceutical formulations by the proposed method and by the pharmacopoeia reference procedure.
