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Inhibition of erythrocyte ghost ATPase by polyene antibiotics

E Capuozzo1, S Jullien, C Salerno

  • 1Department of Biochemical Sciences, University of Rome, Italy.

Biochemistry International
|January 1, 1990
PubMed

Insights

Polyene antibiotics like amphotericin B affect erythrocyte ghost ATPase activities. Specific antibiotics inhibited (Mg2+)-ATPase and (Na+ + K+ + Mg2+)-ATPase, while (Ca2+ + Mg2+)-ATPase showed minimal impact.

Area of Science:

  • Biochemistry
  • Membrane Biology
  • Pharmacology

Background:

  • Erythrocyte membranes contain various ATPase enzymes crucial for cellular function.
  • Polyene antibiotics are known to interact with cell membranes, potentially affecting protein activity.

Purpose of the Study:

  • To investigate the impact of micromolar concentrations of polyene antibiotics on erythrocyte ghost ATPase activities.
  • To determine the differential effects of specific polyene antibiotics on different ATPase isoforms.

Main Methods:

  • Isolation of erythrocyte ghosts.
  • Assay of ATPase activities in the presence of varying polyene antibiotic concentrations.
  • Enzyme kinetic analysis.

Main Results:

  • (Mg2+)-ATPase activity was significantly inhibited by amphotericin B and amphotericin B methyl ester.
  • (Na+ + K+ + Mg2+)-ATPase activity was inhibited by amphotericin B and lucensomycin.
  • (Ca2+ + Mg2+)-ATPase activity exhibited only slight sensitivity to the tested polyene antibiotics.

Conclusions:

  • Polyene antibiotics exert differential inhibitory effects on erythrocyte ghost ATPase enzymes.
  • Amphotericin B demonstrates broad inhibitory action across multiple ATPase isoforms.
  • The findings provide insights into the membrane-interactive mechanisms of polyene antibiotics.

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