Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Study of electroreflectance spectrum and Franz-Keldysh effect at metal-GaAs interfaces].

Guang pu xue yu guang pu fen xi = Guang pu·2008
Same author

[Study on electro-degradation of new conjugated polymer PFO-BT15 light emitting diodes].

Guang pu xue yu guang pu fen xi = Guang pu·2008
Same author

Comparison of the curative effects of video assisted thoracoscopic anterior correction and small incision, thoracotomic anterior correction for idiopathic thoracic scoliosis.

Chinese medical journal·2008
Same author

Distribution and sources of mercury in soils from former industrialized urban areas of Beijing, China.

Environmental monitoring and assessment·2008
Same author

[Main flavonoids from Sophora flavescenes].

Yao xue xue bao = Acta pharmaceutica Sinica·2008
Same author

External validation and prediction employing the predictive squared correlation coefficient test set activity mean vs training set activity mean.

Journal of chemical information and modeling·2008
Same journal

Long-term cryostorage does not impair human sperm motility: evidence from a three-decade cohort study.

Journal of assisted reproduction and genetics·2026
Same journal

Donor versus partner sperm in IVF/ICSI: risk of preeclampsia and obstetric outcomes in a prospective registry-linkage study.

Journal of assisted reproduction and genetics·2026
Same journal

Long-term maternal health outcomes following autologous in vitro fertilization compared with medically unassisted conceptions and with nulliparas.

Journal of assisted reproduction and genetics·2026
Same journal

Fertility preservation with successful pregnancy outcome in a young patient with endometrial cancer.

Journal of assisted reproduction and genetics·2026
Same journal

Recombinant LH supplementation during ovarian stimulation modulates early embryo morphokinetics without affecting IVF outcomes in normal responders: a retrospective time-lapse study.

Journal of assisted reproduction and genetics·2026
Same journal

Redefining success in assisted reproductive technologies: the Embryo-Maternal-Offspring Continuum Framework.

Journal of assisted reproduction and genetics·2026
See all related articles

Related Experiment Video

Updated: Jun 1, 2026

Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing
09:16

Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing

Published on: October 11, 2015

Differentially expressed micoRNAs in human oocytes.

Yan-Wen Xu1, Bin Wang, Chen-Hui Ding

  • 1Reproductive Medical Center, the First Affiliated Hospital of Sun Yat-sen University, No 58, Zhongshan Rd 2, Guangzhou, China.

Journal of Assisted Reproduction and Genetics
|June 8, 2011
PubMed
Summary
This summary is machine-generated.

This study identified specific microRNAs (miRNAs) that change during human oocyte meiosis. High FSH levels in in vitro maturation medium altered the expression of key miRNAs, impacting oocyte development.

More Related Videos

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay
08:00

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay

Published on: June 16, 2021

Using Mouse Oocytes to Assess Human Gene Function During Meiosis I
11:13

Using Mouse Oocytes to Assess Human Gene Function During Meiosis I

Published on: April 10, 2018

Related Experiment Videos

Last Updated: Jun 1, 2026

Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing
09:16

Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing

Published on: October 11, 2015

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay
08:00

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay

Published on: June 16, 2021

Using Mouse Oocytes to Assess Human Gene Function During Meiosis I
11:13

Using Mouse Oocytes to Assess Human Gene Function During Meiosis I

Published on: April 10, 2018

Area of Science:

  • Reproductive biology
  • Molecular genetics
  • Developmental biology

Background:

  • MicroRNAs (miRNAs) play crucial roles in gene regulation.
  • Oocyte maturation is a complex meiotic process essential for female reproduction.
  • Understanding miRNA dynamics during oogenesis is vital for reproductive medicine.

Purpose of the Study:

  • To identify differentially expressed microRNAs (miRNAs) during human oocyte meiosis.
  • To characterize the expression patterns of specific miRNAs from the germinal vesicle (GV) to the metaphase II (MII) stage.
  • To investigate the impact of FSH concentration on miRNA expression during in vitro maturation (IVM).

Main Methods:

  • miRNA microarray analysis of GV and MII oocytes.
  • Real-time RT-PCR to validate miRNA expression changes.
  • Oocyte culture under conventional and high FSH concentrations for IVM.

Main Results:

  • Four miRNAs (hsa-miR-193a-5p, hsa-miR-297, hsa-miR-625, hsa-miR-602) were upregulated, and eleven miRNAs were downregulated in MII oocytes compared to GV oocytes.
  • RT-PCR confirmed dynamic expression changes for hsa-miR-15a and hsa-miR-20a from GV to MII stages.
  • High FSH concentration during IVM reversed the expression trends of hsa-miR-15a and hsa-miR-20a.

Conclusions:

  • Specific miRNAs exhibit dynamic expression changes throughout human oocyte meiosis.
  • High FSH levels during IVM can significantly alter miRNA expression profiles.
  • These findings contribute to understanding molecular regulation of oocyte maturation and may inform fertility treatments.