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qPCR Is a Sensitive and Rapid Method for Detection of Cytomegaloviral DNA in Formalin-fixed, Paraffin-embedded Biopsy Tissue
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qPCR Is a Sensitive and Rapid Method for Detection of Cytomegaloviral DNA in Formalin-fixed, Paraffin-embedded Biopsy Tissue

Published on: July 9, 2014

Precision across the analytical measuring range of a quantitative real-time PCR assay for cytomegalovirus detection

Thomas E Grys1, Doreen L Duquette, Bruce White

  • 1Molecular Microbiology Laboratory, Mayo Medical Laboratories New England, 160 Dascomb Rd., Andover, MA 01810, USA. grys.thomas@mayo.edu

Journal of Clinical Microbiology
|June 10, 2011
PubMed

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...