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Hyperthyroidism II: Pathophysiology

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Related Experiment Video

Updated: Jun 1, 2026

An Ex vivo Culture System to Study Thyroid Development

An Ex vivo Culture System to Study Thyroid Development

Published on: June 6, 2014

Evaluation of proliferation potential in thyroid normo-/hypofunctioning and hyperfunctioning nodules.

Marioara Cornianu¹, V Stan, Elena Lazăr

¹Department of Pathology, Victor BabesUniversity of Medicine and Pharmacy, Timisoara, Romania. marioaracornianu@yahoo.com

Romanian Journal of Morphology and Embryology = Revue Roumaine De Morphologie Et Embryologie

|June 10, 2011

Summary

Thyroid nodules show increased cell proliferation compared to normal tissue, particularly in follicular adenomas and adenomatous nodules. This heightened proliferation is a common feature in toxic thyroid nodules, regardless of their specific pathology.

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Last Updated: Jun 1, 2026

An Ex vivo Culture System to Study Thyroid Development

An Ex vivo Culture System to Study Thyroid Development

Published on: June 6, 2014

Area of Science:

Endocrinology
Oncology
Pathology

Background:

Thyroid nodules, including follicular adenomas (FA) and adenomatous nodules (AN), are common, especially in iodine-deficient regions.
These nodules can be functionally classified as normo-/hypofunctioning (scintigraphically cold - SCN) or hyperfunctioning (scintigraphically hot - SHN).

Purpose of the Study:

To evaluate the proliferation potential in thyroid nodules (SCN and SHN) using tissue samples from surgically treated patients.
To compare proliferation markers between different types of thyroid nodules and surrounding normal thyroid tissue.

Main Methods:

Immunohistochemical analysis of Proliferating Cell Nuclear Antigen (PCNA) and Ki-67 in 20 SCN (8 FA, 12 AN) and 16 toxic nodules (6 FA, 10 toxic multinodular goiters).
Calculation of Proliferation Index (PI-PCNA and PI-Ki-67) for nodules and adjacent normal thyroid tissue.
Statistical evaluation using the unpaired t-test.

Main Results:

Significantly higher PI-PCNA observed in FA and AN compared to normal thyroid tissue (p<0.029 and p<0.001, respectively).
PI-PCNA was significantly higher in FA than AN (p<0.01).
Increased proliferation rate (PI-Ki-67) was noted in nodules with lymphocytic thyroiditis and in toxic thyroid nodules (TTN) compared to normal tissue.

Conclusions:

Increased thyrocyte proliferation in thyroid nodules contributes to the clinical presentation of SCN.
Elevated proliferative potential is a common characteristic of toxic thyroid nodules, irrespective of their histological subtype.