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The Ras Gene

The Ras Gene

The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a superfamily...

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Updated: Jun 1, 2026

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Published on: September 25, 2018

A fully integrated, automated and rapid detection system for KRAS mutations.

Norio Ureshino¹, Naoko Sueoka-Aragane, Tomomi Nakamura

¹Division of Hematology, Respiratory Medicine and Oncology, Department of Internal Medicine, Faculty of Medicine, Saga University, and Department of Medical Oncology, Saga Prefectural Hospital, Saga 849-8501, Japan.

Oncology Reports

|June 14, 2011

Summary

A new automated quenching probe (QP) method accurately and rapidly detects KRAS mutations in lung adenocarcinoma. This system offers a faster alternative to conventional methods for targeted therapy prediction.

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Fully Processed Recombinant KRAS4b: Isolating and Characterizing the Farnesylated and Methylated Protein

Published on: January 16, 2020

Area of Science:

Oncology
Molecular Biology
Genetics

Background:

KRAS mutations are prevalent in various cancers and indicate resistance to EGFR-targeted therapies.
Accurate and rapid KRAS mutation detection is vital for cancer screening and predicting treatment efficacy.

Purpose of the Study:

To develop and validate a novel, automated quenching probe (QP) method for KRAS mutation detection.
To compare the performance of the QP method against the conventional direct sequencing (DS) method.

Main Methods:

Genotyping of KRAS mutations in 136 lung adenocarcinoma patients using both direct sequencing (DS) and the automated quenching probe (QP) method.
Assessing the detection limit and sensitivity of the QP method using control plasmids.

Main Results:

The QP method achieved automated data acquisition within 60 minutes.
The QP method demonstrated a detection limit of 50 copies and could detect 10% mutant plasmid in mixtures.
The QP and DS methods yielded concordant results in 134 out of 136 cases; the QP method identified two samples as positive that were negative by DS, likely due to lower cancer cell content.

Conclusions:

The quenching probe (QP) method is a highly accurate and rapid system for detecting KRAS mutations.
This automated system shows potential for improving KRAS mutation analysis in clinical settings, aiding in personalized cancer therapy.