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Updated: Jun 1, 2026

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy
Published on: May 16, 2022
Three-dimensional high-resolution imaging of gold nanorods uptake in sentinel lymph nodes
Yeongri Jung1, Roberto Reif, Yaguang Zeng
1Department of Bioengineering, University of Washington, 3720 15th Avenue NE, Seattle, Washington 98195, United States.
Abstract:
In this paper, we demonstrate an application of a noninvasive imaging modality, photothermal optical coherence tomography (PT-OCT), for imaging gold nanorods (GNRs) uptake in sentinel lymph node (SLN) of mice in situ. This application enables us to obtain higher quality images of SLN structures due to the photothermal contrast properties of the GNRs. It is also demonstrated that GNRs accumulate differently within several SLN structures, and this uptake is time dependent. Finally, we determine the average concentration of GNRs within the whole SLN which is used to demonstrate uptake kinetics of the nanoparticles.

