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Characterization of the transcripts of human cytomegalovirus UL144

Rong He1, Yanping Ma, Ying Qi

  • 1Clinical Genetics Department, The Affiliated Shengjing Hospital, China Medical University, 110004 Shenyang, Liaoning of China.

Virology Journal
|June 15, 2011
PubMed
Abstract

Insights

Researchers identified multiple human cytomegalovirus (HCMV) UL144 gene transcripts in infants. The 1600 nucleotide transcript was the most abundant form, revealing complex UL144 gene transcription in clinical HCMV isolates.

Area of Science:

  • Virology
  • Molecular Biology
  • Genetics

Background:

  • Human cytomegalovirus (HCMV) genome research, especially the UL/b' region.
  • Identification of UL144 gene transcripts in 3 clinical HCMV isolates from congenitally infected infants.

Purpose of the Study:

  • To investigate the transcriptional complexity of the HCMV UL144 gene.
  • Characterize UL144 transcripts in clinical isolates.

Main Methods:

  • Northern blot hybridization.
  • cDNA library screening.
  • Rapid Amplification of Complementary DNA (RACE-PCR).

Main Results:

  • At least 4 differentially regulated 3'-coterminal UL144 transcripts (1,300, 1,600, 1,700, and 3,500 nucleotides) were identified.
  • The 1,600 nucleotide transcript was the predominant UL144 mRNA.
  • The largest transcript encompassed UL141, UL142, UL143, UL144, and UL145 open reading frames (ORFs), initiating within the UL141 ORF.

Conclusions:

  • The study demonstrates the intricate transcriptional regulation of the UL144 gene in HCMV.
  • Findings highlight the complexity of viral gene expression in clinical settings.

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