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OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy
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Protein stabilization in a highly knotted protein polymer.

Tobias C Sayre1, Toni M Lee, Neil P King

  • 1Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, USA.

Protein Engineering, Design & Selection : PEDS
|June 15, 2011
PubMed
Summary

Protein knots, while rare, can enhance protein stability. Polymerizing carbonic anhydrase II created knotted filaments, revealing that deep knots significantly stabilize proteins against structural loss and aggregation.

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