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Immunogold Electron Microscopy

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

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Related Experiment Video

Updated: May 31, 2026

Preparation and Photoacoustic Analysis of Cellular Vehicles Containing Gold Nanorods

Preparation and Photoacoustic Analysis of Cellular Vehicles Containing Gold Nanorods

Published on: May 2, 2016

Surface-modified gold nanoshells for enhanced cellular uptake.

Zhongshi Liang¹, Yun Liu, Xiangyang Li

¹Institute for Advanced Interdisciplinary Research, East China Normal University, Shanghai, China.

Journal of Biomedical Materials Research. Part A

|June 18, 2011

Summary

This summary is machine-generated.

Modifying gold nanoshells with meso-2,3-dimercaptosuccinic acid (DMSA) significantly enhances their cellular uptake. This surface modification improves nanoparticle delivery for biomedical applications.

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Area of Science:

Biomedical Engineering
Nanotechnology
Cell Biology

Background:

Gold nanoshells show promise for biomedical applications.
Efficient cellular delivery of nanoparticles is crucial for many applications.
Surface modification can influence nanoparticle-cell interactions.

Purpose of the Study:

To develop a method for enhancing gold nanoshell cellular uptake.
To investigate the effect of meso-2,3-dimercaptosuccinic acid (DMSA) modification on gold nanoshell uptake.
To elucidate the mechanism of enhanced cellular uptake.

Main Methods:

Modification of gold nanoshells with DMSA.
Quantification of cellular uptake using inductively coupled plasma atomic emissions spectroscopy (ICP-AES).
Microscopic analysis using transmission electron microscopy (TEM).
In vitro studies with endocytic inhibitors across three cell lines (RAW 264.7, A549, BEL-7402).

Main Results:

DMSA-modified gold nanoshells exhibited significantly enhanced cellular uptake compared to unmodified ones.
Enhanced uptake is potentially due to non-specific protein adsorption on DMSA-modified nanoshells.
Cellular internalization of DMSA-modified gold nanoshells occurred via receptor-mediated endocytosis (RME).

Conclusions:

Surface modification of gold nanoshells with DMSA is an effective strategy to improve cellular uptake.
The enhanced uptake mechanism involves protein adsorption and RME.
This finding offers valuable insights for nanoparticle design in biomedical applications.