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Updated: May 31, 2026

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation
Published on: June 21, 2016
A transcription factor hijacking to regulate RARα by using a chimeric molecule of retinoic acid and a DNA alkylator
Kyung Hoon Min1, Byoung-Gu Yun, Yoonsung Lee
1College of Pharmacy, Chung-Ang University, Seoul 156-756, Republic of Korea.
Abstract:
As a model compound for the transcription factor hijacking mechanism of action of DNA damaging agent that simultaneously bind to the nuclear receptor, we designed and synthesized a chimeric molecule, RA-mustard, which can bind with both retinoic acid receptor α (RARα) and DNA. The interaction between RA-mustard with RARα was confirmed by binding assay using RARα-overexpressing cell extract. RA-mustard-modified DNA diminished the RARα-dependent luciferase expression in the RARα-abundant cells.
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