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Related Concept Videos

¹H NMR Signal Integration: Overview00:58

¹H NMR Signal Integration: Overview

The intensity of a signal, which can be represented by the area under the peak, depends on the number of protons contributing to that signal. The area under each peak is shown as a vertical line called an integral, with the integral value listed under it, as seen in the proton NMR spectrum of benzyl acetate. Each integral value is divided by the smallest integral value to obtain the ratio of the number of protons producing each signal. The ratio reveals the relative number of protons and not...
Applications Of NMR In Biology01:25

Applications Of NMR In Biology

Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
The...
Single-pass Transmembrane Proteins01:25

Single-pass Transmembrane Proteins

Integral membrane proteins are tightly associated with the cell membrane and play a crucial role in cell communication, signaling, adhesion, and transport of the molecules. Some integral membrane proteins are present only in the membrane monolayer. For example, the enzyme fatty acid amide hydrolase is present in the cytoplasmic side of the membrane monolayer. In contrast, another type of integral membrane protein, also known as a transmembrane protein, spans across the membrane. Transmembrane...
Introduction to Membrane Proteins01:16

Introduction to Membrane Proteins

The cell membrane, or plasma membrane, is an ever-changing landscape. It is described as a fluid mosaic where various macromolecules are embedded in the phospholipid bilayer. Among the macromolecules are proteins. The protein content varies across cell types. For example, mitochondrial inner membranes contain ~76% protein content, while myelin contains ~18% protein content. Individual cells contain many types of membrane proteins—red blood cells contain over 50—and different cell types have...

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Related Experiment Video

Updated: May 31, 2026

Exploring Protein-Glycan Interactions: Advances in Nuclear Magnetic Resonance
10:07

Exploring Protein-Glycan Interactions: Advances in Nuclear Magnetic Resonance

Published on: August 26, 2025

Solution NMR study of integral membrane proteins.

CongBao Kang1, Qingxin Li

  • 1Experimental Therapeutics Centre, Agency for Science, Technology and Research, Singapore 138669, Singapore. cbkang@etc.a-star.edu.sg

Current Opinion in Chemical Biology
|June 21, 2011
PubMed
Summary

Solution NMR spectroscopy advances integral membrane protein studies. This technique offers unique insights for structural analysis and drug discovery of these vital cell signaling proteins.

Related Experiment Videos

Last Updated: May 31, 2026

Exploring Protein-Glycan Interactions: Advances in Nuclear Magnetic Resonance
10:07

Exploring Protein-Glycan Interactions: Advances in Nuclear Magnetic Resonance

Published on: August 26, 2025

Area of Science:

  • Biochemistry
  • Structural Biology
  • Biophysics

Background:

  • Membrane proteins are crucial for cell signaling and are key drug targets.
  • Obtaining structural information on membrane proteins is challenging due to expression, purification, and membrane-mimicking system difficulties.

Purpose of the Study:

  • To review recent advancements in solution Nuclear Magnetic Resonance (NMR) spectroscopy for integral membrane protein structural studies.
  • To highlight the potential of solution NMR in membrane protein structural analysis and drug discovery.

Main Methods:

  • Solution NMR spectroscopy applied to integral membrane proteins.
  • Discussion of recent advances and examples demonstrating the technique's utility.

Main Results:

  • Solution NMR is effective for determining the structure of integral membrane proteins.
  • Recent advances have overcome previous limitations in applying NMR to these challenging molecules.

Conclusions:

  • Solution NMR spectroscopy is a powerful and unique tool for integral membrane protein structural studies.
  • This technique holds significant promise for advancing drug discovery efforts targeting membrane proteins.