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Updated: May 31, 2026

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High Sensitivity 5-hydroxymethylcytosine Detection in Balb/C Brain Tissue
Published on: February 1, 2011
Discrimination between 5-hydroxymethylcytosine and 5-methylcytosine by a chemically designed peptide
Akiko Nomura1, Kaori Sugizaki, Hiroyuki Yanagisawa
1Advanced Science Institute, RIKEN, Wako, Saitama 351-0198, Japan.
Abstract:
An artificial phosphopeptide recognized the difference between methylated and hydroxymethylated cytosines in DNA. The Sp1 zinc finger peptide substituted by phosphotyrosine effectively discriminated between 5-methylcytosine, 5-hydroxymethylcytosine ((hm)C) and unmethylated cytosine. The DNA recognition properties of the peptide differ from those of other chemicals that detect (hm)C.

