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Updated: May 31, 2026

17:12
Profiling of Methyltransferases and Other S-adenosyl-L-homocysteine-binding Proteins by Capture Compound Mass Spectrometry (CCMS)
Published on: December 20, 2010
Activity-Based Protein Profiling (ABPP) and Click Chemistry (CC)-ABPP by MudPIT Mass Spectrometry
Anna E Speers1, Benjamin F Cravatt
1The Skaggs Institute for Chemical Biology and Department of Physiological Chemistry, The Scripps Research Institute, La Jolla, CA.
Current Protocols in Chemical Biology
|June 25, 2011
Abstract
None:
Activity-based protein profiling (ABPP) is a chemical proteomic method for functional interrogation of enzymes within complex proteomes. This Unit presents a protocol for in vitro and in vivo labeling using ABPP and Click Chemistry (CC)-ABPP probes for in-depth profiling using the Multi-dimensional Protein Identification Technology (MudPIT) analysis platform.

