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Updated: May 31, 2026

Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization

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Published on: October 6, 2019

Polymeric immunoglobulin receptor.

Masatake Asano¹, Kazuo Komiyama

¹Department of Pathology, Nihon University School of Dentistry, Tokyo, Japan. asano-m@dent.nihon-u.ac.jp

Journal of Oral Science

|June 30, 2011

Summary

The mucosal immune system uses dimeric IgA (dIgA) to protect the gut. Intestinal epithelial cells transport dIgA via the polymeric immunoglobulin receptor (pIgR) to form secretory IgA (SIgA).

Area of Science:

Immunology
Cell Biology
Gastroenterology

Background:

The intestinal tract relies on the mucosal immune system for protection and homeostasis.
Epithelial cells and lymphocytes cooperate within this system.
Polymeric immunoglobulins (pIgs), especially dimeric IgA (dIgA), are key players.

Purpose of the Study:

To review the transport mechanism of dIgA across intestinal epithelial cells.
To highlight the role of the polymeric immunoglobulin receptor (pIgR) in this process.
To discuss recent advancements in understanding mucosal immunity.

Main Methods:

Review of existing literature on dIgA transport and pIgR function.
Analysis of the molecular mechanisms involved in transcytosis.

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Last Updated: May 31, 2026

Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization

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A Protocol for the Production of KLRG1 Tetramer

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Antibody Binding Specificity for Kappa (Vκ) Light Chain-containing Human (IgM) Antibodies: Polysialic Acid (PSA) Attached to NCAM as a Case Study

Published on: June 29, 2016

Synthesis of current knowledge on secretory IgA (SIgA) formation.

Main Results:

Dimeric IgA (dIgA) is captured by pIgR on the basolateral surface of intestinal epithelial cells (IECs).
The dIgA-pIgR complex undergoes transcytosis across IECs.
Proteolytic cleavage on the apical surface generates secretory IgA (SIgA).

Conclusions:

The pIgR-mediated transcytosis is crucial for delivering dIgA to the intestinal lumen.
This process is essential for maintaining gut homeostasis and pathogen defense.
Ongoing research continues to elucidate the complexities of mucosal immune responses.