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Specific PCR to identify the heavy-metal-resistant bacterium Cupriavidus metallidurans
Michael P Ryan1, Catherine C Adley
1Microbiology Laboratory, Department of Chemical and Environmental Sciences, University of Limerick, Limerick, Ireland.
Abstract:
The aim of this study is to develop a polymerase chain reaction (PCR) assay for rapid detection of Cupriavidus metallidurans. PCR primers targeting the Signal transduction histidine kinase gene were designed and designated Cm-F1/Cm-R1. Strains of C. metallidurans were positively identified. The size of the PCR products was 437 bp, as expected. This PCR method enables monitoring of industrial, environmental and clinical sources for presence of C. metallidurans.
