On the histochemistry of prostaglandin-dehydrogenase

Using PGF2alpha as substrate the PGDH-activity was localised in the male and in the female rat kidney by histochemical technique. The reaction is independent of the presence of NAD, but with NAD the reaction intensity is enhanced. The effectors phenazine methosulphate, Triton X 100 and Tween 80 showed no enhancing effect on the enzyme activity. In the rat kidney we have found the reaction product predominantly in the inner cortex (straight portion of the proximal tubules), however, sex dependent differences of PGDH-activity and localization are visible.