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[Effects of CD74 gene on IFNγR gene expression in MG TEC]
Qian-Ru Li1, Wen-Qiao Zang, Feng Gao
1Department of Microbiology and Immunology, Zhengzhou University, Zhengzhou, China. liruqian@yahoo.com.cn
Aim:
To study the effects of CD74 gene on IFN-γR expression in myasthenia gravis thymic epithelial cell(TEC).
Methods:
PCMV-CD74 transiently transfect to primary cultured TEC mediated with lipofectamine, the result of transfection and some autoimmune related genes such as IFN-γR were detected by real-time RT-PCR.
Results:
Real-time quantitative RT-PCR results show that the mRNA expression level of IL-4R, IL-32, IFN-γR, ECGF1, HLA-A, HLA-DR increased significantly, with the high express ion of CD74 gene in thymic epithelial cells.
Conclusion:
CD74 gene over-expression in TEC can increase IFN-γR mRNA expression.