[A preliminary study on the effect of lincomycin on the immune function of dendritic cell line DC2.4]

To explore the effect of lincomycin (lin) on the immune function of dendritic cell (DCs) line DC2.4.

Three experimental groups, namely, DC2.4 cells group, DC2.4 Cells+LPS group and DC2.4 cells +LPS +lin group were established (LPS and lin were 500 ng/mL). The morphological changes in each group were observed under inverted microscope. The MHC class II , CD86 and CD80 on the DC2.4 cells were detected by flow cytometer. The effects of lipopolysaccharide (LPS) and LPS +lin on the DC2.4 cells immuno-stimulatory capacity were evaluated by allogeneic mixed leukocytes reaction (MLR) between DC2.4 cells and T cells. ELISA was adopted to quantitate the level of IFN-γin the supernatant of cultured DC2.4 cells and T cells in each group.

DC2.4 cells showed typical morphology of immature DCs. When stimulated with LPS or LPS combined with lin, DC2.4 cells exhibited typical maturate DCs modality. LPS of 500 ng/mL could significantly up-regulate the expression of MHC class II , CD86 and CD80 on DC2.4 cells and also augment stimulatory action of DC2.4 cells on T cells proliferation and secretion of IFN-γ. However, compared with LPS alone, the treatment of lin (500 ng/mL) combined with LPS down-regulated the immmuno-regulation function of DC2.4 cells.

Lin can partly inhibit the immuno-regulation function of maturate DC2.4 cells.