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Updated: May 31, 2026
Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood
Published on: February 6, 2018
[Development and characterization of monoclonal antibody against human FcR γ chain]
Kun Qian1, Wen-jie Jin, Hong-xia Shao
1Yangzhou University, Yangzhou, China. qianku@yzu.edu.cn
Aim:
The current study was aimed to generate mouse monoclonal antibodies (mAbs) specific for Fc receptor common γ chain (FcRγ), which serves a critical signaling molecule for numerous human immune receptors that play essential roles in human immune responses.
Methods:
Two BALB/c mice were immunized with the synthesized polypeptides containing 19 residues at the carboxyl terminus of human FcR γ chain. The spleen cells from the immunized mice were fused with myeloma Sp2/0 cells. ELISA was used to screen mAbs and the positive clones were selected for further characterization with Western blot and flow cytometry assays.
Results:
After screen, there hybridoma cell lines which secreted mAbs specifically recognized the FcR γ chain were obtained and named as 5B6, 7D3 and 8D4. The 7D3 mAb had the best quality and it could recognize the FcR γ chain in vivo and in vitro with 2.5 μg/mL.
Conclusion:
We have generated the FcRγ-specific mAbs, which may serve as a valuable tool in the functional studies of FcRγ associated immune receptors.
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