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Ozone postconditioning in renal ischaemia-reperfusion model. Functional and morphological evidences.
A Fernández Iglesias1, L González Núñez, J L Calunga Fernández
1Departamento de Investigaciones, Instituto de Nefrología Dr. Abelardo Buch, Ave. 26 y Ave. Rancho Boyeros, La Habana, Cuba. aimeefdez@infomed.sld.cu
Summary
Ozone postconditioning protects kidneys from ischaemia-reperfusion injury. This study found ozone therapy improved renal function and reduced tissue damage in a rat model, offering a potential therapeutic strategy.
Area of Science:
- Nephrology
- Renal Physiology
- Oxidative Stress Biology
Background:
- Ischaemia-reperfusion injury is a primary cause of acute kidney complications, notably acute tubular necrosis.
- Ozone therapy, through oxidative preconditioning, modulates renal cell redox state by enhancing antioxidant mechanisms.
- Ischaemic postconditioning has also demonstrated similar protective effects in kidney injury models.
Purpose of the Study:
- To investigate the protective effects of ozone oxidative postconditioning on kidney function and morphology.
- To assess the efficacy of ozone therapy in an established rat model of renal ischaemia-reperfusion injury.
Main Methods:
- Forty female Wistar rats were allocated into four groups: negative control, positive control, oxygen treatment, and ozone treatment.
- The positive control, oxygen, and ozone groups underwent 60 minutes of renal ischaemia followed by 10 days of reperfusion.
- Ozone (0.5 mg/kg) or oxygen (26 mg/kg) was administered rectally during the reperfusion phase; renal function and histology were evaluated.
Main Results:
- The ozone group exhibited no significant changes in filtration fraction or proteinuria compared to the negative control.
- Glomerular filtration rate, renal plasma flow, and creatinine levels showed modest improvements in the ozone group versus oxygen and positive control groups.
- Histological examination revealed significantly less overall kidney damage in the ozone group compared to the oxygen and positive control groups.
Conclusions:
- Ozone postconditioning demonstrates a protective effect on renal function following ischaemia-reperfusion.
- The findings suggest that ozone therapy can preserve kidney morphology and function, indicating its therapeutic potential.
