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Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...

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Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay
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Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay

Published on: May 25, 2015

Mobile microRNAs hit the target.

Nial R Gursanscky1, Iain R Searle, Bernard J Carroll

  • 1School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia.

Traffic (Copenhagen, Denmark)
|July 23, 2011
PubMed
Summary
This summary is machine-generated.

MicroRNAs (miRNAs) move between plant cells, regulating gene expression for development. This intercellular movement is also crucial for animal development, highlighting a conserved biological mechanism.

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Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells

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Last Updated: May 30, 2026

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay
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Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
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Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells
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Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells

Published on: September 28, 2011

Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Plant Science

Background:

  • MicroRNAs (miRNAs) are key gene expression regulators in eukaryotes.
  • Small interfering RNAs (siRNAs) mediate cellular defense mechanisms.
  • A central question is whether miRNAs function only locally or move between cells.

Purpose of the Study:

  • To investigate the intercellular movement of microRNAs (miRNAs) in plants.
  • To determine if miRNAs play a role in developmental programming through cell-to-cell transport.
  • To explore the implications of miRNA movement for both plant and animal development.

Main Methods:

  • Analysis of gene expression gradients in plant tissues.
  • Investigating the role of miRNA transport in differentiation.
  • Reviewing evidence for intercellular miRNA movement in animals.

Main Results:

  • Recent findings confirm that miRNAs can move between plant cells.
  • Intercellular miRNA movement establishes essential gene expression gradients for development.
  • Evidence suggests similar miRNA movement occurs in animal development.

Conclusions:

  • Intercellular movement of miRNAs is a significant mechanism in plant development.
  • This mobility of miRNAs is likely conserved and crucial for animal development.
  • Understanding miRNA transport offers insights into conserved developmental processes across kingdoms.