Related Experiment Video
Updated: Aug 16, 2026

Generation of Enterobacter sp. YSU Auxotrophs Using Transposon Mutagenesis
Published on: October 31, 2014
Excision of transposon Tn917 in Bacillus subtilis
1Zentralinstitut für Genetik und Kulturpflanzenforschung, Akademie der Wissenschaften der DDR, Gatersleben.
Abstract:
Excision of Tn917 from chromosomal sites in B. subtilis is characterized by reversion studies. We propose different modes of excision depending on the site of transposon insertion. Excision takes place as precise excision in one step which results in reversion of the mutant phenotype, or by a two-step process where nearly precise excision of the transposon moiety is followed by precise excision of the nearly precise excision remnants. For this type of transposons a minor pathway or nonreplicative transposition is proposed to be connected with precise excision.
Related Concept Videos
Overview of Transposition and Recombination
DNA-only Transposons
The donor site from where the transposon is excised is either degraded or...
Conservative Site-specific Recombination and Phase Variation
The recognition sites for Cre recombinase called LoxP...
Transposons

