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Updated: May 30, 2026

Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry

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Published on: March 9, 2018

Pulsed Nano-Electrospray Ionization: Characterization of Temporal Response and Implementation with a Flared Inlet

Jared M Bushey¹, Desmond A Kaplan, Ryan M Danell

¹Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599-3290, USA.

Instrumentation Science & Technology

|July 26, 2011

Summary

Researchers investigated the temporal response of pulsed nano-electrospray ionization mass spectrometry (nano-ESI-MS). They characterized ion formation and detection, achieving a maximum pulse rate of 12 Hz with a custom high-voltage pulsing circuit.

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Spatial Separation of Molecular Conformers and Clusters

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Last Updated: May 30, 2026

Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry

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Published on: March 9, 2018

Spatial Separation of Molecular Conformers and Clusters

Spatial Separation of Molecular Conformers and Clusters

Published on: January 9, 2014

Area of Science:

Analytical Chemistry
Mass Spectrometry

Background:

Pulsed nano-electrospray ionization mass spectrometry (nano-ESI-MS) offers potential for enhanced detection.
Understanding its temporal response is crucial for optimizing ion formation and signal acquisition.

Purpose of the Study:

To characterize the temporal response of pulsed nano-ESI-MS.
To evaluate the influence of pulsing on ion formation and detection.
To demonstrate a controllable multi-sprayer pulsed nano-ESI source.

Main Methods:

Utilized a custom-built high-voltage pulsing circuit to control a multi-sprayer nano-ESI source.
Measured the temporal response (rise and decay times) of mass-resolved ion current.
Investigated ion formation and detection characteristics under pulsed operation.

Main Results:

Determined ion current rise time to be 20 ± 3 msec and decay time to be 61 ± 5 msec.
Achieved a maximum pulse rate of 12 Hz.
Demonstrated that source operation mode (pulsed or continuous) is controllable via applied voltage.

Conclusions:

The temporal characteristics of pulsed nano-ESI-MS were successfully defined.
A controllable pulsed nano-ESI source was developed, enabling flexible operation modes.
This work provides insights for optimizing pulsed nano-ESI-MS applications.