Related Experiment Video
Updated: May 30, 2026
High-throughput Detection Method for Influenza Virus
Published on: February 4, 2012
[The verification of a new DNA double-detection diagnostic kit for identifying novel influenza A (H1N1)]
Wei Wang1, Li-ming Liu, Tong-sheng Guo
1The Department of Clinical Medical Laboratory, the 4th People's Hospital of Chengdu, Chengdu 610016, China.
Objective:
To verify a new kit of "universal and novel influenza A (H1N1) virus nucleic acid double-detection methods (PCR-fluorescence probe)".
Methods:
150 cases of throat swab specimens were collected consecutively. After RNA was extracted, the specimens were detected by the verified kit. At the same time, the same specimens were detected by Real-time PCR diagnostic kit from Beijing CDC as the control. The data were analysed by the Kappa in agreement and by McNemar chi2 in difference test.
Results:
The consistency rate of the verified kit and the Beijing CDC kit was universal primer M 97.33%, H1N1 98.67% respectively. The Kappa test and McNemar chi2 test showed that two methods had a higher consistency. Compared to the CDC kit, the "false negative rate" and "false-positive rate"of double-check kit were lower.
Conclusion:
The kit of "universal and novel influenza A (H1N1) virus nucleic acid double-detection methods (PCR-fluorescence probe)" from Shanghai Kehua Bio-Engineering Co., Ltd can be used to detect influenza A and novel influenza A (H1N1).
