Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: May 30, 2026

Multiple-mouse Neuroanatomical Magnetic Resonance Imaging

Multiple-mouse Neuroanatomical Magnetic Resonance Imaging

Published on: February 27, 2011

Quantitative pharmacologic MRI in mice.

Teodora-Adriana Perles-Barbacaru¹, Daniel Procissi, Andrey V Demyanenko

¹Biological Imaging Center, Beckman Institute, California Institute of Technology, Pasadena, CA 91125, USA.

NMR in Biomedicine

|July 28, 2011

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Quality Assurance Strategies for Brain State Characterization by MEMRI.

bioRxiv : the preprint server for biology·2026

Same author

Induction of ferroptotic and amyloidogenic signatures linked to Alzheimer's disease by chemically distinct air pollutants.

bioRxiv : the preprint server for biology·2026

Same author

Reconfiguration of brain-wide neural activity after early life adversity.

Proceedings of the National Academy of Sciences of the United States of America·2025

Same author

Characterization of experimental cerebral malaria by volumetric MRI A comparative study across the sexes.

PloS one·2025

Same author

Self-assembling dendrimer nanodrug formulations for decreased hERG-related toxicity and enhanced therapeutic efficacy.

Science advances·2025

Same author

Liganded magnetic nanoparticles for magnetic resonance imaging of α-synuclein.

NPJ Parkinson's disease·2025

Same journal

Liver Diffusion Weighted MRI: Effect of Iron Overload on Apparent Diffusion Coefficient.

NMR in biomedicine·2026

Same journal

In Vivo Assessment of Placental Structure and Perfusion in Late-Gestation Pregnancies and Their Association With Fetal Growth.

NMR in biomedicine·2026

Same journal

Reproducibility of Splanchnic Blood Flow Measured Using Phase-Contrast MRI.

NMR in biomedicine·2026

Same journal

Restriction-Weighted Q-Space Trajectory Imaging (ResQ): Toward Mapping Diffusion-Time Effects With Tensor-Valued Diffusion Encoding in Human Prostate Cancer Xenografts.

NMR in biomedicine·2026

Same journal

In Vivo Quantitative Detection of PEGylated Macromolecules by Magnetic Resonance Spectroscopy.

NMR in biomedicine·2026

Same journal

Metabolic Assessment in Human Pluripotent Stem Cell-Derived Cerebral Organoids Using HR-MAS NMR Spectroscopy.

NMR in biomedicine·2026

See all related articles

Pharmacologic MRI (phMRI) effectively measures drug effects on brain hemodynamics using ultrasmall superparamagnetic iron oxide (USPIO) agents. This technique reveals drug-induced changes in cerebral blood volume (CBV), offering insights into brain activity responses.

Area of Science:

Neuroimaging
Pharmacology
Biophysics

Background:

Pharmacologic MRI (phMRI) noninvasively measures drug-induced hemodynamic changes in vivo.
Cerebral blood volume change (ΔCBV) is a surrogate for neuronal activity via neurovascular coupling.
phMRI aids in understanding drug effects on brain activity and behavioral phenotypes in mutant mouse studies.

Purpose of the Study:

To evaluate three intravascular ultrasmall superparamagnetic iron oxide (USPIO) contrast agents for phMRI.
To assess the utility of USPIOs for cerebral blood volume (CBV)-weighted phMRI experiments.
To investigate the hemodynamic responses to cocaine and acetazolamide using phMRI.

Main Methods:

Gradient-echo phMRI technique with 1-minute temporal resolution at high magnetic field (7 T).

More Related Videos

A Mouse Model for Pathogen-induced Chronic Inflammation at Local and Systemic Sites

A Mouse Model for Pathogen-induced Chronic Inflammation at Local and Systemic Sites

Published on: August 8, 2014

MRI and PET in Mouse Models of Myocardial Infarction

MRI and PET in Mouse Models of Myocardial Infarction

Published on: December 19, 2013

Related Experiment Videos

Last Updated: May 30, 2026

Multiple-mouse Neuroanatomical Magnetic Resonance Imaging

Multiple-mouse Neuroanatomical Magnetic Resonance Imaging

Published on: February 27, 2011

A Mouse Model for Pathogen-induced Chronic Inflammation at Local and Systemic Sites

A Mouse Model for Pathogen-induced Chronic Inflammation at Local and Systemic Sites

Published on: August 8, 2014

MRI and PET in Mouse Models of Myocardial Infarction

MRI and PET in Mouse Models of Myocardial Infarction

Published on: December 19, 2013

Evaluation of three USPIO contrast agents, including assessment of tissue half-life and r(2)/r(1) ratios.

Determination of optimal echo-time for functional contrast to noise ratio (fCNR) and assessment of contrast agent stability.

Main Results:

Three USPIOs demonstrated suitable properties (r(2)/r(1) ≥ 20, half-life ≥ 1.5 h) for CBV-weighted phMRI.
An echo-time of ~10 ms or longer yielded fCNR > 75, with stable contrast between 1.5-2 h post-injection.
Cocaine challenge reduced CBV in mouse brain, primarily in the nucleus accumbens; acetazolamide increased blood volume globally.

Conclusions:

USPIO contrast agents are effective for phMRI, enabling robust CBV measurements.
phMRI can detect specific regional and global hemodynamic changes induced by different pharmacological agents.
This study validates USPIO-enhanced phMRI for investigating drug-induced neurovascular and neurochemical alterations.