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[Relation between gastric dendritic cells and H pylori-associated gastritis]

Han-bing Ning1, Yan-hong Wang, Lian-feng Zhang

  • 1Department of Digestive Deseases, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi = Chinese Journal of Cellular and Molecular Immunology
|August 3, 2011
PubMed
Abstract

Insights

Gastric dendritic cells (DCs) increase with Helicobacter pylori infection and inflammation, suggesting a role in early gastritis. This study isolated gastric DCs to understand their function in H. pylori-associated gastritis.

Area of Science:

  • Immunology
  • Gastroenterology
  • Microbiology

Background:

  • Helicobacter pylori infection is a common cause of gastritis.
  • Gastric immune responses, particularly involving dendritic cells (DCs), are crucial in H. pylori-associated gastritis.
  • The specific role of gastric DCs in the early stages of this condition requires further elucidation.

Purpose of the Study:

  • To investigate the effects and quantity of gastric dendritic cells (DCs) in patients with H. pylori-associated gastritis.
  • To explore the correlation between gastric DC levels and H. pylori density or the degree of gastric inflammation.

Main Methods:

  • Mononuclear cells were isolated from gastric biopsies of healthy subjects and gastritis patients.
  • Human Leukocyte Antigen - antigen D Related (HLA-DR) positive dendritic cells (DCs) were isolated using magnetic-activated cell sorting (MACS).
  • H. pylori presence was confirmed using rapid urease test, Warthin-Starry stain, and C14-urea breath test.

Main Results:

  • Gastric mononuclear cells from H. pylori-infected mucosa showed a significantly higher percentage of HLA-DR+ DCs (17.93%) compared to non-infected subjects (4.93%).
  • The quantity of gastric DCs positively correlated with H. pylori density.
  • Gastric DC levels were significantly elevated in moderate and high inflammatory gastric mucosa compared to low inflammatory mucosa.

Conclusions:

  • Gastric dendritic cells (DCs) were successfully isolated from gastric samples.
  • The findings suggest that DCs play a significant role in the early pathogenesis of H. pylori-associated gastritis.

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