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Updated: May 30, 2026

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
09:39

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Published on: December 20, 2014

FBXW5 controls centrosome number

Julia Pagan, Michele Pagano

    Nature Cell Biology
    |August 3, 2011
    PubMed

    Insights

    FBXW5 controls the degradation of HsSAS-6, a key factor in centriole assembly. This study reveals FBXW5 as a target of PLK4 and APC/C, crucial for regulating centriole duplication.

    Area of Science:

    • Cell Biology
    • Molecular Biology
    • Genetics

    Background:

    • Centriole duplication is essential for cell division, but the precise regulatory mechanisms preventing overduplication remain unclear.
    • The centriole assembly factor HsSAS-6 plays a critical role in initiating the formation of new centrioles.

    Discussion:

    • This study identifies FBXW5 as a key regulator that targets HsSAS-6 for degradation, thereby preventing centriole overduplication.
    • FBXW5 itself is proposed to be a substrate of PLK4 (Polo-like kinase 4) and the Anaphase-Promoting Complex/Cyclosome (APC/C).
    • These findings place FBXW5 within the established regulatory network of centriole duplication, involving PLK4 and APC/C.

    Key Insights:

    • FBXW5 mediates the degradation of the centriole assembly factor HsSAS-6.
    • FBXW5 acts as a crucial link in the regulatory pathway controlling centriole duplication.
    • The study implicates FBXW5 in the coordinated action of PLK4 and APC/C to ensure proper centriole numbers.

    Outlook:

    • Further investigation into the precise interactions between FBXW5, PLK4, and APC/C will elucidate the complete mechanism of centriole duplication control.
    • Understanding these regulatory pathways could offer insights into developmental disorders and cancers associated with centriole abnormalities.

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