Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Dual Immunofluorescence of γH2AX and 53BP1 in Human Peripheral Lymphocytes05:34

Dual Immunofluorescence of γH2AX and 53BP1 in Human Peripheral Lymphocytes

2.2K
This protocol presents a method to assess the formation and repair of DNA double-strand breaks through the simultaneous detection of γH2AX and 53BP1 foci in interphase nuclei of bleomycin-treated human peripheral...
2.2K
Sensitivity Enhancement of Soft Capacitive Pressure Sensors Using a Solvent Evaporation-Based Porosity Control Technique10:28

Sensitivity Enhancement of Soft Capacitive Pressure Sensors Using a Solvent Evaporation-Based Porosity Control Technique

2.4K
A simple and cost-efficient fabrication method based on the solvent evaporation technique is presented to optimize the performance of a soft capacitive pressure sensor, which is enabled by porosity control in the dielectric layer using different mass ratios of the molding PDMS/toluene...
2.4K
Immunofluorescence Microscopy of γH2AX and 53BP1 for Analyzing the Formation and Repair of DNA Double-strand Breaks10:47

Immunofluorescence Microscopy of γH2AX and 53BP1 for Analyzing the Formation and Repair of DNA Double-strand Breaks

17.2K
This manuscript provides a protocol for the analysis of DNA double-strand breaks by immunofluorescence microscopy of γH2AX and 53BP1.
17.2K
Nuclear Stability03:18

Nuclear Stability

22.9K
Protons and neutrons, collectively called nucleons, are packed together tightly in a nucleus. With a radius of about 10−15 meters, a nucleus is quite small compared to the radius of the entire atom, which is about 10−10 meters. Nuclei are extremely dense compared to bulk matter, averaging 1.8 × 1014 grams per cubic centimeter. If the earth’s density were equal to the average nuclear density, the earth’s radius would be only about 200 meters.
To hold positively charged protons together...
22.9K
RNA Stability01:53

RNA Stability

35.6K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
35.6K
Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in Primary Alveolar Epithelial Cells10:49

Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in Primary Alveolar Epithelial Cells

6.5K
Here, we present a tool that can be used to study the posttranscriptional modulation of a transcript in primary alveolar epithelial cells by using an inducible expression system coupled to a pipette electroporation...
6.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cell autonomous inflammation in VEXAS is mediated by cGAS-STING.

bioRxiv : the preprint server for biology·2026
Same author

Clonal dynamics of germinal center refueling by secondary immunization.

bioRxiv : the preprint server for biology·2026
Same author

Replaying germinal center evolution on a quantified affinity landscape.

Cell·2026
Same author

Bound for the nucleus: defining the molecular principles of cargo selection by importin 9.

bioRxiv : the preprint server for biology·2026
Same author

Pan-cancer proteogenomic interrogation of the Ubiquitin Proteasome System.

bioRxiv : the preprint server for biology·2026
Same author

Effects of thymidylate synthase inhibitors differ in genomic uracilation and mutagenic potential.

Life science alliance·2026

Related Experiment Video

Updated: Jan 20, 2026

Dual Immunofluorescence of γH2AX and 53BP1 in Human Peripheral Lymphocytes
05:34

Dual Immunofluorescence of γH2AX and 53BP1 in Human Peripheral Lymphocytes

Published on: July 14, 2023

2.2K

GMCL1 controls 53BP1 stability and modulates taxane sensitivity.

Yuki Kito1,2, Tania J González-Robles1,3,4, Sharon Kaisari1,2,4

  • 1Department of Biochemistry and Molecular Pharmacology, New York University Grossman School of Medicine, New York, United States.

Elife
|January 19, 2026
PubMed
Summary
This summary is machine-generated.

GMCL1 regulates the mitotic stopwatch pathway (MSP) by degrading 53BP1, preventing p53 stabilization. Its depletion sensitizes paclitaxel-resistant cancers, offering a potential therapeutic target for improving cancer treatment.

Keywords:
53BP1GMCL1cancer biologycell biologyhumanmitotic stopwatchp53prolonged mitosisprotein degradation

More Related Videos

Sensitivity Enhancement of Soft Capacitive Pressure Sensors Using a Solvent Evaporation-Based Porosity Control Technique
10:28

Sensitivity Enhancement of Soft Capacitive Pressure Sensors Using a Solvent Evaporation-Based Porosity Control Technique

Published on: March 24, 2023

2.4K
Immunofluorescence Microscopy of γH2AX and 53BP1 for Analyzing the Formation and Repair of DNA Double-strand Breaks
10:47

Immunofluorescence Microscopy of γH2AX and 53BP1 for Analyzing the Formation and Repair of DNA Double-strand Breaks

Published on: November 3, 2017

17.2K

Related Experiment Videos

Last Updated: Jan 20, 2026

Dual Immunofluorescence of γH2AX and 53BP1 in Human Peripheral Lymphocytes
05:34

Dual Immunofluorescence of γH2AX and 53BP1 in Human Peripheral Lymphocytes

Published on: July 14, 2023

2.2K
Sensitivity Enhancement of Soft Capacitive Pressure Sensors Using a Solvent Evaporation-Based Porosity Control Technique
10:28

Sensitivity Enhancement of Soft Capacitive Pressure Sensors Using a Solvent Evaporation-Based Porosity Control Technique

Published on: March 24, 2023

2.4K
Immunofluorescence Microscopy of γH2AX and 53BP1 for Analyzing the Formation and Repair of DNA Double-strand Breaks
10:47

Immunofluorescence Microscopy of γH2AX and 53BP1 for Analyzing the Formation and Repair of DNA Double-strand Breaks

Published on: November 3, 2017

17.2K

Area of Science:

  • Cell Biology
  • Molecular Oncology
  • Cancer Therapeutics

Background:

  • Mitotic surveillance pathways ensure proper cell division by monitoring mitosis duration.
  • Prolonged mitosis activates the mitotic stopwatch pathway (MSP) complex (53BP1, USP28, p53), stabilizing p53 and inducing cell cycle arrest or apoptosis.
  • Paclitaxel resistance in cancer involves bypassing mitotic surveillance, but mechanisms are unclear.

Purpose of the Study:

  • Identify novel regulators of mitotic surveillance.
  • Investigate the role of GMCL1 in the MSP pathway and its implications in paclitaxel resistance.

Main Methods:

  • Protein interaction studies to map 53BP1 and GMCL1 binding domains.
  • Ubiquitin ligase assays to assess CRL3GMCL1 activity on 53BP1.
  • Gene depletion experiments (GMCL1, 53BP1, USP28) in cell cycle arrest models.
  • Assessment of paclitaxel sensitivity in cancer cells with varying GMCL1 and p53 levels.

Main Results:

  • GMCL1 directly interacts with 53BP1 and targets it for degradation via CRL3GMCL1 during M phase.
  • GMCL1 depletion impairs cell cycle progression after mitotic arrest, a phenotype rescued by silencing 53BP1 or USP28.
  • GMCL1 depletion sensitizes cancer cells to paclitaxel in a p53-dependent manner.
  • Dysregulated GMCL1 leads to reduced 53BP1 and p53 levels, promoting proliferation.

Conclusions:

  • GMCL1 acts as a negative regulator of the MSP pathway by promoting 53BP1 degradation.
  • Impaired GMCL1-mediated 53BP1 degradation contributes to paclitaxel resistance by preventing p53 activation.
  • Targeting GMCL1 may overcome paclitaxel resistance in p53-proficient cancers.