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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

Confocal Fluorescence Microscopy

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

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Updated: May 30, 2026

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

Wide-field high-resolution structured illumination solid immersion fluorescence microscopy.

Lin Wang¹, Mark C Pitter, Michael G Somekh

¹Institute of Biophysics Imaging and Optical Science, The University of Nottingham, University Park, Nottingham NG7 2RD, UK.

|August 3, 2011

Summary

This summary is machine-generated.

Structured illumination solid immersion fluorescence microscopy (SISIM) combines aplanatic solid immersion lenses and structured illumination microscopy for high-resolution imaging. This novel technique achieves a wide field of view with a numerical aperture equivalent to NA 3.

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Last Updated: May 30, 2026

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Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

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Published on: August 15, 2014

Area of Science:

Optical Microscopy
Biophotonics
Superresolution Imaging

Background:

Aplanatic solid immersion lenses (ASILs) enhance resolution in optical microscopy.
Structured illumination microscopy (SIM) increases spatial bandwidth for higher resolution.

Purpose of the Study:

To investigate the synergistic combination of ASILs and SIM in fluorescence microscopy.
To develop a wide-field, high-resolution microscopic system.
To achieve a numerical aperture (NA) beyond conventional limits.

Main Methods:

Integration of ASILs with SIM in a fluorescence microscope setup.
Development of structured illumination solid immersion fluorescence microscopy (SISIM).
Characterization of the system's resolution and field of view.

Main Results:

Demonstration of a wide-field high-resolution microscopic system using SISIM.
Achieved a system bandwidth corresponding to a numerical aperture (NA) of 3.
Validated the potential for significantly enhanced lateral resolution.

Conclusions:

SISIM offers a powerful approach for achieving both wide-field imaging and high resolution.
The combined technique overcomes limitations of individual methods.
Future work can further enhance the resolution capabilities of SISIM.