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Updated: May 30, 2026

Myosin-Specific Adaptations of In vitro Fluorescence Microscopy-Based Motility Assays
Published on: February 4, 2021
Functional surface attachment in a sandwich geometry of GFP-labeled motor proteins
Volker Bormuth1, Felix Zörgibel, Erik Schäffer
1Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
Abstract:
Molecular motors perform work in cells by moving in an ATP-dependent manner along filamentous tracks. In vitro, the mechanical action of such motor proteins can be investigated by attaching the molecules to surfaces in the so-called gliding or bead assays. Surface attachment protocols have to be used that do not interfere with the function of the molecule. Here, we describe a sandwich protocol that preserves functionality. The protocol can be used for a large variety of proteins, in particular kinesin motor proteins that are GFP-tagged.
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