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Updated: May 30, 2026

Studying the Epithelial Effects of Intestinal Inflammation In Vitro on Established Murine Colonoids
Published on: June 2, 2023
Stem cells and inflammation in the intestine
Adam Humphries1, Trevor A Graham, Stuart A C McDonald
1Histopathology Unit, London Research Institute, Cancer Research UK, London, UK.
Intestinal stem cell research is advancing rapidly. Recent studies reveal niche succession and monoclonal conversion in human colon crypts, expanding our understanding of gut stem cell dynamics.
Area of Science:
- Gastroenterology and Stem Cell Biology
- Human Intestinal Physiology
Background:
- Intestinal stem cell biology is a rapidly evolving field.
- Specific markers like Lgr5 and Bmi1 have identified stem cells in murine models.
- Limited research exists on human intestinal stem cells.
Purpose of the Study:
- To investigate stem cell dynamics within the human colon.
- To explore processes like niche succession and monoclonal conversion in human crypts.
- To understand crypt division and expansion in the human colon.
Main Methods:
- Analysis of human colon tissue.
- Identification and tracking of stem cell populations.
- Histological and molecular characterization of crypt structures.
Main Results:
- Niche succession, where one stem cell replaces others, occurs in human crypts.
- Monoclonal conversion, leading to clonal crypt populations, is observed in humans.
- Human colon crypts demonstrate capacity for division and expansion.
Conclusions:
- Human intestinal stem cell behavior shares similarities with murine models.
- Processes like niche succession and monoclonal conversion are key to human gut homeostasis.
- Understanding these dynamics is crucial for regenerative medicine and disease research.
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07:45Ultrasound-guided Intracardiac Injection of Human Mesenchymal Stem Cells to Increase Homing to the Intestine for Use in Murine Models of Experimental Inflammatory Bowel Diseases
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