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Related Experiment Video

Updated: May 30, 2026

Derivation of Human Embryonic Stem Cells by Immunosurgery
11:56

Derivation of Human Embryonic Stem Cells by Immunosurgery

Published on: December 13, 2007

Embryonic stem cell derivation from human embryos.

Paul Lerou1

  • 1Department of Newborn Medicine, Brigham & Women's Hospital/Harvard Medical School, Harvard Institutes of Medicine, Boston, MA, USA. plerou@partners.org

Methods in Molecular Biology (Clifton, N.J.)
|August 9, 2011
PubMed
Summary
This summary is machine-generated.

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Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
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Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
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Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
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This study details a protocol for deriving human embryonic stem cells (hESCs) from embryos. The research focuses on optimizing hESC derivation from blastocyst-stage embryos for regenerative medicine applications.

Area of Science:

  • Stem cell biology
  • Developmental biology
  • Regenerative medicine

Background:

  • Human embryonic stem cells (hESCs) are pluripotent and self-renewing, offering significant potential for research and regenerative medicine.
  • hESCs are typically derived from excess or discarded human embryos created during in vitro fertilization (IVF).
  • While derivation is possible from earlier embryonic stages, blastocyst-stage embryos yield the highest efficiency.

Purpose of the Study:

  • To describe a detailed protocol for deriving human embryonic stem cell lines.
  • To optimize the derivation of pluripotent stem cells from human embryos.

Main Methods:

  • Utilizing embryos, particularly those at the blastocyst stage, for stem cell isolation.
  • Employing established cell culture techniques for pluripotent stem cell line establishment.

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Derivation of Stem Cell Lines from Mouse Preimplantation Embryos
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Derivation of Stem Cell Lines from Mouse Preimplantation Embryos

Published on: August 20, 2017

Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction
10:47

Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

Published on: October 28, 2011

Related Experiment Videos

Last Updated: May 30, 2026

Derivation of Human Embryonic Stem Cells by Immunosurgery
11:56

Derivation of Human Embryonic Stem Cells by Immunosurgery

Published on: December 13, 2007

Derivation of Stem Cell Lines from Mouse Preimplantation Embryos
12:59

Derivation of Stem Cell Lines from Mouse Preimplantation Embryos

Published on: August 20, 2017

Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction
10:47

Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

Published on: October 28, 2011

  • Describing step-by-step procedures for hESC derivation.

    • Main Results:

    • Successful derivation of human embryonic stem cell lines from human embryos.
    • Demonstration that blastocyst-stage embryos are optimal for efficient hESC derivation.
    • Protocol provides a reproducible method for generating pluripotent stem cells.

    Conclusions:

    • The described protocol enables the derivation of valuable human embryonic stem cell lines.
    • This method supports the advancement of regenerative medicine through stem cell research.
    • Efficient derivation protocols are crucial for harnessing the therapeutic potential of hESCs.